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pp38 antibody #4511  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc pp38 antibody #4511
    HTMCs (n=3) were pretreted with PBA, NaB, or NaAc followed by ZIKV (Z) (PRVABC59 strain, MOI: 1) infection for 48h. Mock-treated cells were used as controls. The cell lysates from mock, ZIKV-infected, and drug-treated cells were subjected to western blotting for the NFκB, ERK1/2, <t>p38,</t> STAT1, STAT2, STST3, RIG-I, IRF3, and IFIT2 pathways. (B) Densitometric analysis of the immunoblots was performed using ImageJ. The bar graph represents means ± SD from three biological replicates. * P < 0.05; ** P < 0.005; *** P < 0.0005; ****, P < 0.0001 (one-way ANOVA).
    Pp38 Antibody #4511, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pp38 antibody #4511/product/Cell Signaling Technology Inc
    Average 90 stars, based on 1 article reviews
    pp38 antibody #4511 - by Bioz Stars, 2026-02
    90/100 stars

    Images

    1) Product Images from "Gut microbial metabolites butyrate and acetate limit Zika virus replication and associated ocular manifestations via the G-protein coupled receptor 43/FFAR2"

    Article Title: Gut microbial metabolites butyrate and acetate limit Zika virus replication and associated ocular manifestations via the G-protein coupled receptor 43/FFAR2

    Journal: bioRxiv

    doi: 10.1101/2025.07.15.664962

    HTMCs (n=3) were pretreted with PBA, NaB, or NaAc followed by ZIKV (Z) (PRVABC59 strain, MOI: 1) infection for 48h. Mock-treated cells were used as controls. The cell lysates from mock, ZIKV-infected, and drug-treated cells were subjected to western blotting for the NFκB, ERK1/2, p38, STAT1, STAT2, STST3, RIG-I, IRF3, and IFIT2 pathways. (B) Densitometric analysis of the immunoblots was performed using ImageJ. The bar graph represents means ± SD from three biological replicates. * P < 0.05; ** P < 0.005; *** P < 0.0005; ****, P < 0.0001 (one-way ANOVA).
    Figure Legend Snippet: HTMCs (n=3) were pretreted with PBA, NaB, or NaAc followed by ZIKV (Z) (PRVABC59 strain, MOI: 1) infection for 48h. Mock-treated cells were used as controls. The cell lysates from mock, ZIKV-infected, and drug-treated cells were subjected to western blotting for the NFκB, ERK1/2, p38, STAT1, STAT2, STST3, RIG-I, IRF3, and IFIT2 pathways. (B) Densitometric analysis of the immunoblots was performed using ImageJ. The bar graph represents means ± SD from three biological replicates. * P < 0.05; ** P < 0.005; *** P < 0.0005; ****, P < 0.0001 (one-way ANOVA).

    Techniques Used: Infection, Western Blot

    IFNAR1 −/− mice (n=6-8) were pretreated with FFAR2 inhibitor 4-CMTB followed by PBA, NaB, or NaAc via i.p. administration, and ZIKV infection as per the timeline shown in . Seven days post-infection, anterior segment/TM tissue from treated and untreated mice were harvested and subjected to (A) RNA extraction and qPCR to measure the mRNA expression levels of PRRs (RIG-I, TLR3), inflammatory (IL-6, IL-1β, CCL-4), IFNs (IFN-α2, IFN-β1), and ISGs (ISG15, OAS2, MX1), and (B) western blot for NFκB, MAPKs (ERK1/2, p38), STAT1, STAT3, RIG-I, and IRF7 inflammatory/ISG pathways. (C) Densitometric analysis of the immunoblots was performed using ImageJ. The bar graph represents means ± SD from three biological replicates. * P < 0.05; ** P < 0.005; *** P < 0.0005; ****, P < 0.0001 (one-way ANOVA).
    Figure Legend Snippet: IFNAR1 −/− mice (n=6-8) were pretreated with FFAR2 inhibitor 4-CMTB followed by PBA, NaB, or NaAc via i.p. administration, and ZIKV infection as per the timeline shown in . Seven days post-infection, anterior segment/TM tissue from treated and untreated mice were harvested and subjected to (A) RNA extraction and qPCR to measure the mRNA expression levels of PRRs (RIG-I, TLR3), inflammatory (IL-6, IL-1β, CCL-4), IFNs (IFN-α2, IFN-β1), and ISGs (ISG15, OAS2, MX1), and (B) western blot for NFκB, MAPKs (ERK1/2, p38), STAT1, STAT3, RIG-I, and IRF7 inflammatory/ISG pathways. (C) Densitometric analysis of the immunoblots was performed using ImageJ. The bar graph represents means ± SD from three biological replicates. * P < 0.05; ** P < 0.005; *** P < 0.0005; ****, P < 0.0001 (one-way ANOVA).

    Techniques Used: Infection, RNA Extraction, Expressing, Western Blot



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    CBD treatment promote activation of markers of MAPK pathway in HPV-positive HNSCC cells. Western blot analysis of MAPK pathway markers like <t>p38,</t> ERK1/2, JNK/SAPK and MK2 and it’s signaling after treatment with 10 μM of CBD in HPV-positive HNSCC cells (A) UPCI: SCC154 and (B) UD-SCC-2 for 30 minutes.
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    Image Search Results


    HTMCs (n=3) were pretreted with PBA, NaB, or NaAc followed by ZIKV (Z) (PRVABC59 strain, MOI: 1) infection for 48h. Mock-treated cells were used as controls. The cell lysates from mock, ZIKV-infected, and drug-treated cells were subjected to western blotting for the NFκB, ERK1/2, p38, STAT1, STAT2, STST3, RIG-I, IRF3, and IFIT2 pathways. (B) Densitometric analysis of the immunoblots was performed using ImageJ. The bar graph represents means ± SD from three biological replicates. * P < 0.05; ** P < 0.005; *** P < 0.0005; ****, P < 0.0001 (one-way ANOVA).

    Journal: bioRxiv

    Article Title: Gut microbial metabolites butyrate and acetate limit Zika virus replication and associated ocular manifestations via the G-protein coupled receptor 43/FFAR2

    doi: 10.1101/2025.07.15.664962

    Figure Lengend Snippet: HTMCs (n=3) were pretreted with PBA, NaB, or NaAc followed by ZIKV (Z) (PRVABC59 strain, MOI: 1) infection for 48h. Mock-treated cells were used as controls. The cell lysates from mock, ZIKV-infected, and drug-treated cells were subjected to western blotting for the NFκB, ERK1/2, p38, STAT1, STAT2, STST3, RIG-I, IRF3, and IFIT2 pathways. (B) Densitometric analysis of the immunoblots was performed using ImageJ. The bar graph represents means ± SD from three biological replicates. * P < 0.05; ** P < 0.005; *** P < 0.0005; ****, P < 0.0001 (one-way ANOVA).

    Article Snippet: Antibodies used in this study were purchased from the following sources: 4G2 (GeneTex, #GTX57154), NS3 (GeneTex, #GTX133309), β-actin (Millipore Sigma, #A2228), pNFκB (#3033), NFκB (#6956) pERK1/2 (#4370), ERK (#4695), pP38 (#4511), P38 (#8690), pSTAT1 (#9167), STAT1 (#14994), pSTAT2 (#88410), STAT2 (#72604), pSTAT3 (#9145), STAT3 (#9139), RIG-I (#3743), pIRF3 (#79945), IRF3 (#4302), and IFIT2 (#92633) antibodies were purchased from Cell Signaling Technology (Danvers, MA).

    Techniques: Infection, Western Blot

    IFNAR1 −/− mice (n=6-8) were pretreated with FFAR2 inhibitor 4-CMTB followed by PBA, NaB, or NaAc via i.p. administration, and ZIKV infection as per the timeline shown in . Seven days post-infection, anterior segment/TM tissue from treated and untreated mice were harvested and subjected to (A) RNA extraction and qPCR to measure the mRNA expression levels of PRRs (RIG-I, TLR3), inflammatory (IL-6, IL-1β, CCL-4), IFNs (IFN-α2, IFN-β1), and ISGs (ISG15, OAS2, MX1), and (B) western blot for NFκB, MAPKs (ERK1/2, p38), STAT1, STAT3, RIG-I, and IRF7 inflammatory/ISG pathways. (C) Densitometric analysis of the immunoblots was performed using ImageJ. The bar graph represents means ± SD from three biological replicates. * P < 0.05; ** P < 0.005; *** P < 0.0005; ****, P < 0.0001 (one-way ANOVA).

    Journal: bioRxiv

    Article Title: Gut microbial metabolites butyrate and acetate limit Zika virus replication and associated ocular manifestations via the G-protein coupled receptor 43/FFAR2

    doi: 10.1101/2025.07.15.664962

    Figure Lengend Snippet: IFNAR1 −/− mice (n=6-8) were pretreated with FFAR2 inhibitor 4-CMTB followed by PBA, NaB, or NaAc via i.p. administration, and ZIKV infection as per the timeline shown in . Seven days post-infection, anterior segment/TM tissue from treated and untreated mice were harvested and subjected to (A) RNA extraction and qPCR to measure the mRNA expression levels of PRRs (RIG-I, TLR3), inflammatory (IL-6, IL-1β, CCL-4), IFNs (IFN-α2, IFN-β1), and ISGs (ISG15, OAS2, MX1), and (B) western blot for NFκB, MAPKs (ERK1/2, p38), STAT1, STAT3, RIG-I, and IRF7 inflammatory/ISG pathways. (C) Densitometric analysis of the immunoblots was performed using ImageJ. The bar graph represents means ± SD from three biological replicates. * P < 0.05; ** P < 0.005; *** P < 0.0005; ****, P < 0.0001 (one-way ANOVA).

    Article Snippet: Antibodies used in this study were purchased from the following sources: 4G2 (GeneTex, #GTX57154), NS3 (GeneTex, #GTX133309), β-actin (Millipore Sigma, #A2228), pNFκB (#3033), NFκB (#6956) pERK1/2 (#4370), ERK (#4695), pP38 (#4511), P38 (#8690), pSTAT1 (#9167), STAT1 (#14994), pSTAT2 (#88410), STAT2 (#72604), pSTAT3 (#9145), STAT3 (#9139), RIG-I (#3743), pIRF3 (#79945), IRF3 (#4302), and IFIT2 (#92633) antibodies were purchased from Cell Signaling Technology (Danvers, MA).

    Techniques: Infection, RNA Extraction, Expressing, Western Blot

    CBD treatment promote activation of markers of MAPK pathway in HPV-positive HNSCC cells. Western blot analysis of MAPK pathway markers like p38, ERK1/2, JNK/SAPK and MK2 and it’s signaling after treatment with 10 μM of CBD in HPV-positive HNSCC cells (A) UPCI: SCC154 and (B) UD-SCC-2 for 30 minutes.

    Journal: Frontiers in Immunology

    Article Title: CBD promotes antitumor activity by modulating tumor immune microenvironment in HPV associated head and neck squamous cell carcinoma

    doi: 10.3389/fimmu.2025.1528520

    Figure Lengend Snippet: CBD treatment promote activation of markers of MAPK pathway in HPV-positive HNSCC cells. Western blot analysis of MAPK pathway markers like p38, ERK1/2, JNK/SAPK and MK2 and it’s signaling after treatment with 10 μM of CBD in HPV-positive HNSCC cells (A) UPCI: SCC154 and (B) UD-SCC-2 for 30 minutes.

    Article Snippet: The primary antibodies p38 (#8690), phospho- p38 (pp38) (#4511), Erk1/2 (#4370), phospho-extracellular signal-regulated kinase (pERK1/2), (#4695), SAPK/JNK (#9252), phospho-c-Jun N-terminal kinase (pJNK) (#4688), MAPKAPK-2 (#3042) and phospho-mitogen-activated-protein-kinase-activated-protein-kinase-2 (pMK-2) (#3007) and Vinculin (#4650) were obtained from Cell Signaling Technology (Danvers, MA, USA) and used at a dilution of 1:1000.

    Techniques: Activation Assay, Western Blot

    CBD treatment promotes colocalization of CD4+T and CD8+T cells along with phospho-p38 (pp38) in the tumor microenvironment of a syngeneic mouse model. (A) Representative images of multiplex IHC analysis to evaluate the staining of CD45, panCK, CD4, CD8 and pp38 MAPK in mice tumor sections obtained from vehicle and CBD-treated groups. (B) Representative images of multiplex IHC analysis to show colocalization of CD4+T cells, CD8+T cells along with pp38 MAPK for the tumor sections obtained from CBD-treated mice group. (C-F) Graphical representation of % of co-localization of panCK:pp38, pp38:CD4+T Cells, pp38:CD8+T Cells and pp38:CD4+T and CD8+T Cells respectively. Statistical analysis was performed by unpaired Student’s t-test [*p<0.05 **p<0.01].

    Journal: Frontiers in Immunology

    Article Title: CBD promotes antitumor activity by modulating tumor immune microenvironment in HPV associated head and neck squamous cell carcinoma

    doi: 10.3389/fimmu.2025.1528520

    Figure Lengend Snippet: CBD treatment promotes colocalization of CD4+T and CD8+T cells along with phospho-p38 (pp38) in the tumor microenvironment of a syngeneic mouse model. (A) Representative images of multiplex IHC analysis to evaluate the staining of CD45, panCK, CD4, CD8 and pp38 MAPK in mice tumor sections obtained from vehicle and CBD-treated groups. (B) Representative images of multiplex IHC analysis to show colocalization of CD4+T cells, CD8+T cells along with pp38 MAPK for the tumor sections obtained from CBD-treated mice group. (C-F) Graphical representation of % of co-localization of panCK:pp38, pp38:CD4+T Cells, pp38:CD8+T Cells and pp38:CD4+T and CD8+T Cells respectively. Statistical analysis was performed by unpaired Student’s t-test [*p<0.05 **p<0.01].

    Article Snippet: The primary antibodies p38 (#8690), phospho- p38 (pp38) (#4511), Erk1/2 (#4370), phospho-extracellular signal-regulated kinase (pERK1/2), (#4695), SAPK/JNK (#9252), phospho-c-Jun N-terminal kinase (pJNK) (#4688), MAPKAPK-2 (#3042) and phospho-mitogen-activated-protein-kinase-activated-protein-kinase-2 (pMK-2) (#3007) and Vinculin (#4650) were obtained from Cell Signaling Technology (Danvers, MA, USA) and used at a dilution of 1:1000.

    Techniques: Multiplex Assay, Staining